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KMID : 0364819850230040265
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Korean Journal of Microbiology
1985 Volume.23 No. 4 p.265 ~ p.270
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Election Microscopic Observation on Protoplast Fusion of Coryneform Bacteria
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Abstract
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1
Overall procedure of cell fusion between Brevibacteriun: jlavunr and Cwynebaeterium glutanticum was morphologically observed by transmission electron microscopy. Protoplasts formed by treatment of cells with penicillin G and lysozyme in order were released through the pores generated on a certain region of cell walls to be spherical form. When two different protoplasts were met, cell wall and membrane in the contact zone was disappeared and followed by the mutual exchange of cytoplasniic and/or chromosomal materials. Cell wall regeneration speed of the protoplasts fused was slower than that of the non-fused, whereas the size of the former was confirmed as bigger than that of the latter.
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